• Dietary SFAs and ω-6 Fatty Acids Alter Incorporation of ω-3 Fatty Acids into Milk Fat of Lactating CD-1 Mice and Tissues of Offspring

    2 monthes ago - By Journal of Nutrition

    ABSTRACTBackgroundMethods to increase the amount of omega-3 PUFAs in milk are desirable for neonatal health. The n-3 PUFA, α-linolenic acid , can be elongated to EPA and DHA. n-6 PUFAs suppress tissue n-3 PUFA incorporation, but the effect of SFAs is not clear.ObjectivesIn this study, we compared the effects of SFAs and n-6 PUFAs on n-3 PUFA incorporation into milk and tissues of lactating mice and tissues of their offspring.MethodsFemale CD-1 mice were bred at 8 wk of age. All experimental diets included 3% flaxseed oil and were begun on day 8 of lactation: low-fat diet ; high-SFA diet...
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  • Tissue and Circulating Fatty Acids as Biomarkers to Evaluate Long-Term Fat Intake Are Tissue and Sex Dependent in CD-1 Mice

    2 monthes ago - By Journal of Nutrition

    ABSTRACTBackgroundThere is currently no consensus on which tissues are optimal for assessing specific diet-derived fatty acids as biomarkers for long-term dietary studies.ObjectivesThis study measured the content of unique diet-derived FAs from dairy, echium, and fish in tissues (adipose, muscle, liver, erythrocyte membranes, and plasma phospholipids, cholesterol esters, triglycerides, and free fatty acids) after long-term feeding in CD-1 mice.MethodsBeginning at weaning, mice were fed 1 of 4 diets that only differed in FA composition: control fat blend , reflecting the FA profile of the...
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